Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Vitamin B2 analysis example Mobile phase composition: buffer (EDTA50mg/L, sodium heptanesulfonate 1.1g/L, glacial acetic acid, triethylamine to adjust the pH to 3.40±0.02): methanol=86:14 Column brand: Hypersil ODS2 4.6×250 mm Flow rate/flow: 1.0 mL/...

Vitamin B6 analysis example Mobile phase composition: buffer (EDTA50mg/L, sodium heptanesulfonate 1.1g/L, glacial acetic acid, triethylamine to adjust the pH to 3.40±0.02): methanol=86:14 Column brand: Hypersil ODS2 4.6×250 mm Flow rate/flow: 1.0 mL/...

Vitamin B12 analysis example Mobile phase composition: 3% orthophosphoric acid aqueous solution: acetonitrile=26:70 Column brand: Hypersil NH2 4.6×250 mm Flow rate/flow: 1.7 mL/min Detection wavelength: UV361nm Injection volume: 10 µL