Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Oxytetracycline Tetracycline Chlortetracycline Analysis Example Mobile phase composition: acetonitrile/sodium dihydrogen phosphate buffer solution=27/73 Column brand: SinoChrom ODS-BP 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV355n...

Caffeine analysis example Mobile phase composition: acetonitrile: water: acetic acid = 16:83:1 Column brand: SinoChrom ODS-BP 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: 273nm Injection volume: 20 μL

Chromium picolinate analysis example Mobile phase composition: acetonitrile: 0.125mol/L phosphate buffer=75:25 Column brand: Hypersil ODS2 4.6×150 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV254nm Injection volume: 20 μL