P-phenylenediamine aniline benzidine p-toluidine p-chloroaniline

Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer

Column: Hypersil ODS2 5 µm 4.6×250 mm

Flow rate: 1.0 mL/min

Detection: Ex473 nm, Em535 nm

Injection volume: 10 µL

Column temperature: 35 ℃

1. p-phenylenediamine 2. aniline 3. benzidine 4. p-toluidine 5. p-chloroaniline