Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Milk-Amino acid analysis example Mobile phase composition: A: acetonitrile/methanol/water=60/20/20, B: 0.025mol/L sodium acetate (pH=6.5±0.05) Column brand: Elite AAP 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV254nm Injection volum...

Whole milk powder-amino acid analysis example Mobile phase composition: A: acetonitrile/methanol/water=60/20/20, B: 0.025mol/L sodium acetate (pH=6.5±0.05) Column brand: Elite AAP 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV254nm In...

Milk (sodium thiocyanate) analysis example Mobile phase composition: Nitrile/phosphate buffer solution (Accurately measure 2.0mL phosphoric acid in a 1000mL volumetric flask, add water to the mark, shake well, pour it into a 1000mL beaker, adjust the...