Putrescine Cadaverine Spermidine Spermine Histamine

Mobile phase: Mobile phase A: Acetonitrile

Mobile phase B: 20mmol/L ammonium acetate solution

Column: SinoChrom ODS-BP 5µm 4.6×150mm

Flow rate: 1.0mL/min

Detection: Ex473nm, Em535nm

Injection volume: 10µL

Column temperature: 35℃

1- putrescine; 2-cadaverine; 3-spermidine; 4-spermine; 5-histamine