Vitamin B2

Vitamin B2 analysis example

Mobile phase composition: buffer (EDTA50mg/L, sodium heptanesulfonate 1.1g/L, glacial acetic acid, triethylamine to adjust the pH to 3.40±0.02): methanol=86:14

Column brand: Hypersil ODS2 4.6×250 mm

Flow rate/flow: 1.0 mL/min

Detection wavelength: UV267nm

Injection volume: 10 µL