Malachite green

Mobile phase: acetonitrile: ammonium acetate (pH4.5)=80:20

Column: SinoChrom ODS-BP 5 µm 4.6×250 mm

Flow rate: 1.2 mL/min

Detection wavelength: 0~6~16 min

618 nm~588 nm~26 6nm

Injection volume: 20µL

Column temperature: room temperature